Secreted vascular endothelial growth factor (VEGF) levels were detected with a Quantikine® rat VEGF assay (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. VEGF concentration in the SC-CM-DMEM was detected firstly. IECs were cultured in SC-CM-DMEM or DMEM with 2% FBS. The VEGF concentration in the supernatants of the wells was detected by ELISA on days 1, 3, 5, and 7. The medium in all the detected wells was fully replaced 24 h before each time point at which the supernatant was collected and analyzed.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.