IECs were resuspended in concentrated SC-CM-DMEM or DMEM at a density of 1 × 107 cells/ml. A total of 2 μl of medium containing cells was homogeneously mixed with 2 μl of 1% gelatin (Aladdin, Shanghai, China). The mixture was injected into the corneas of eight anesthetized 8-week-old female Sprague-Dawley (SD) rats according to the groups (4 rats per group), which were purchased from the Experimental Center of Southern Medical University (Guangzhou, China). After 12 days, the rats were sacrificed and perfused with waterproof drawing ink (Zhonghua, Shanghai, China) by intracardiac injection. The eyes were fixed with 10% neutralized buffered formalin, and then the corneas were excised to observe angiogenesis. Vascular segments were quantified by the ImageJ 1.4.3 software in random fields in quadruplicate samples.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.