Capillary-like tube formation was detected by using Matrigel (BD Biosciences, San Jose, CA). Liquid Matrigel was placed in a 96-well plate (60 μl/well). IECs or AECs (2 × 104 cells/well) were seeded on Matrigel in 40 μl of SC-CM-DMEM or SC-CM-M199. After incubation for 4 h at 37 °C, phase contrast micrographs were taken. Capillary-like meshes were quantified by the ImageJ 1.4.3 software (National Institutes of Health, USA) in random fields in triplicate wells.

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