Capillary-like tube formation was detected by using Matrigel (BD Biosciences, San Jose, CA). Liquid Matrigel was placed in a 96-well plate (60 μl/well). IECs or AECs (2 × 104 cells/well) were seeded on Matrigel in 40 μl of SC-CM-DMEM or SC-CM-M199. After incubation for 4 h at 37 °C, phase contrast micrographs were taken. Capillary-like meshes were quantified by the ImageJ 1.4.3 software (National Institutes of Health, USA) in random fields in triplicate wells.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.