Confluent SCs in 10-cm dishes were rinsed three times with phosphate-buffered saline (PBS), and 10 ml of basal medium (high-glucose DMEM or M199) without FBS was added. This medium was harvested after 48 h and centrifuged for 5 min at 3000×g. Then, the supernatant was collected as SC-conditioned medium (CM)-DMEM or SC-CM-M199 and stored at − 70 °C until use.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.