After the identities of the cDNA amplification products were confirmed, the sequencing library was constructed using a Library Construction KIT (10× Genomics). First, the cDNA was chemically knocked-out. The cDNA fragment was then cut into 200~300 bp fragments, the cDNA fragments were segmented, and their terminals were repaired and added. The cDNA fragments were then screened. The P7 adapter was connected and introduced into the sample index using PCR amplification. Finally, a sequence library was obtained. Sequencing was performed on an Illumina Hiseq 3000/4000 (Illumina, San Diego, CA, USA) with a 150 bp pair-end run by Quick Biology (Pasadena, CA, USA). A data quality check was performed using the SAV (Illumina). Demultiplexing was performed using the Bcl2fastq2 v 2.17 program (Illumina).

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