Worms were recorded crawling freely on NGM plates which were freshly seeded with a circular patch of 120μl OP50 bacteria in LB medium and left to air dry for 4–6 hours to a matte finish. 6–8 young adult worms from each genotype were then placed using an eyelash on a plate and given 5 minutes to recover from the transfer. The plates were placed on an illuminator table (MBF Bioscience) and recorded for 10–12 minutes at 7.5 frames/second with a resolution of 2592x1944 pixels using an overhanging fixed lens (Nikon AF Micro-Nikkor 60 mm f/2.8D) focused at 10 μm/pixel.

From each video, a section of three minutes was subsequently analyzed using the WormLab software (MBF Bioscience, Williston, VT, USA) to recover average values of relevant parameters such as speed, wavelength and amplitude. Worms were tracked for a minimum of 100 seconds, accounting for short segments of poor tracking, which were manually removed within the software.

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