pLKO.1 lentiviral vectors expressing short hairpin RNAs (shRNAs) targeting RUNX2 (shRUNX2), ABL (shABL), MMP13 (shMMP13) or a nonspecific GFP sequence (shGFP) were co-transfected into HEK293T cells with pPAX2 and pVSVG (Addgene) using X-tremeGENE 9 transfection reagent (Roche). The virus was collected 48 hours after transfection, and cells were infected as described previously (25).

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