All cultures were maintained in a 5% CO2 environment at 37°C. HEK 293T cells (ATCC) were cultured in DMEM (GIBCO) supplemented with 10% fetal bovine serum (FBS) (Sigma). MDA-MB231 cells (ATCC) were cultured in α-MEM (Nacalai Tesque) supplemented with 10% FBS. MDA-MB231 cells stably expressing luciferase were cultured in DMEM/F12 (Gibco) supplemented with 10% FBS. Saos-2 cells (ATCC) were cultured in McCoy’s 5A Modified Medium (GIBCO) supplemented with 15% FBS.

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