Resolution of two peaks (R), defined as the ratio of the difference in retention times between two peaks and the average baseline width of two peaks (Harris, 2010), was determined using Eq. (1):

where TR1 and TR2 are the retention times of adjacent peaks (analyte 1 elutes before analyte 2) and wb1 and wb2 are the widths of the peaks at baseline.

The limit of detection (LOD), defined as the lowest concentration of analyte in a sample that can be readily distinguished from the absence of that analyte (a blank value) (McNaught and Wilkinson, 1997; Inczedy et al., 1998; Allegrini and Olivieri, 2014), was determined using Eq. (2):

The LOQ, defined as the smallest concentration of analyte in a sample that can be quantitatively determined with suitable precision and accuracy, was determined using Eq. (3):

In Eqs. (2) and (3), Sa is the standard deviation of the response estimated by the standard error of y-intercepts of the regression lines and b is the slope of the calibration curve (Shrivastava and Gupta, 2011). A calibration curve with concentrations between 0.3 and 25 μg/L was used to obtain LOD and LOQ of all analytes.

Accuracy, defined as the closeness between a measured value and either a true or accepted value, was evaluated from precision and trueness values of each analyte (Munch et al., 2005). The precision was determined by calculating the relative standard deviation (RSD) using Eq. (4). Trueness was determined by calculating the recovery using Eq. (5).

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