After clinical evaluation by a dermatologist, nail samples with clinically suspected onychomycosis were collected by scraping. This material was sent to the Laboratory of Medical Mycology, UEM, for processing.

Mycological diagnosis was performed in two stages: direct mycological examination with 20% KOH and 0.5% Evans blue to clarify the material and observation of the sample through light microscopy. The samples were also spread on sabouraud dextrose agar (SDA) (Difco™ Detroit) and selective agar for pathogenic fungi (Difco™ Detroit), with nine inoculations in each type of culture medium. The cultures were incubated at 25°C for 30 days to allow fungus growth, with daily evaluation to verify the growth rate. The identification of each isolated fungus considered growth time, uniformity of the colonies grown, macro and micromorphology, and biochemical test results [12]. In both cases, T. rubrum, an anthropophilic dermatophyte fungus, was identified. The isolates were deposited in the Microbial Collections of the Paraná Network (Taxonline) at the Federal University of Paraná as T. rubrum CMRP2912 and T. rubrum CMRP2918, respectively.

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