For the preparation of crude extracts, we have used all solvents, of HPLC grades. In the order of increasing polarities, these were Chloroform (99.9%, Sigma-Aldrich, LiChrosolv, Malaysia), Ethyl acetate, Acetone (99.5% Chemiz, Malaysia), Ethanol, Methanol (99.8%, ChemAR, Systerm, Malaysia) and double distilled Milli-Q Type 1 water (MilliporeMerck, Germany). For LC–MS and GC–MS studies, we have used the solvents of MS grades.

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