Female Balb/c, C57BL/6 and C1qa knockout mice (C1qatmld(EUCOMM)Wtsi) were purchased from Jackson Laboratory (JAX, Bar Harbor, ME, USA). The use of mice for this study was approved by the Institutional Animal Care and Use Committee at the Albert Einstein College of Medicine, protocol 2018-0504. Balb/c or C57BL/6 mice (age 6 weeks) were vaccinated intramuscularly (prime) and then three weeks later (boost) with ΔgD-2, rgD-2/Alum-MPL or a control VD60 lysate, as previously described [12]. Blood was obtained by retro-orbital sampling one week and three weeks post-boost.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.