Female Balb/c, C57BL/6 and C1qa knockout mice (C1qatmld(EUCOMM)Wtsi) were purchased from Jackson Laboratory (JAX, Bar Harbor, ME, USA). The use of mice for this study was approved by the Institutional Animal Care and Use Committee at the Albert Einstein College of Medicine, protocol 2018-0504. Balb/c or C57BL/6 mice (age 6 weeks) were vaccinated intramuscularly (prime) and then three weeks later (boost) with ΔgD-2, rgD-2/Alum-MPL or a control VD60 lysate, as previously described [12]. Blood was obtained by retro-orbital sampling one week and three weeks post-boost.

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