The construction of A+R@PLTs followed the methods reported previously (35). Briefly, 1 × 108 PLTs were mixed with 1 ml of PBS containing 50 μg of Au nanorods. Then, the mixture was treated in an electroporation process under normal conditions for a few seconds. The final A+R@PLTs contained about ~4.5 × 10−9 nmol Au per PLT, which was equal to the concentration of N in N+R@PLTs. The concentration of A+R@PLTs in photothermal effect study in vitro was 1 × 1010 A+R@PLTs/ml (8.8 μg/ml, 44.5 μM for A). For animal experiments, the dose of PLTs and A was 1 × 109 cells per mouse and 0.88 μg per mouse (~4.5 nmol per mouse), respectively.

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