The mouse PLTs were incubated in 0.25× PBS, followed by centrifugation at 9000 rpm for 10 min to obtain the PLT membrane. Meanwhile, the synthesized Au nanorods were incubated with R837 hydrochloride for 1 hour at room temperature to obtain A+R. Last, the PLT membrane and A+R were coextruded through a porous membrane to form A+R@PM. The concentration of A was 8.8 μg/ml (44.5 μM) in photothermal effect in vitro, which was equal to the concentration of N in the same study. The dose of A was 0.9 μg per mouse (~4.5 nmol per mouse) in animal experiment.

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