Mouse whole blood was harvested by retro-orbital puncture and collected in heparinized mouse blood collection tubes. The whole blood was diluted with 2× volume of Hepes medium. The diluted blood was centrifuged for 10 min at 100g, and the collected PLT-rich plasma (PRP) was diluted further in Hepes medium. PRP was centrifuged for 5 min at 2400g, and the white precipitate was pure PLTs.

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