ICSS testing was performed on α-PHP at doses of 0.32, 1.0, and 3.2 mg/kg. These doses were selected following pilot studies that gradually increased dosage in 0.5 log units in individual rats until a behaviorally active dose was identified. ICSS testing was also performed using a 10 mg/kg dose of cocaine and saline as positive and negative controls, respectively. Test sessions consisted of three consecutive baseline components followed by intraperitoneal (i.p.) drug injection and then by pairs of consecutive test components beginning after 10, 30, 100, and 300 min. For 3.2 mg/kg α-PHP, ICSS testing was also performed after 1440 min (24 hr). Treatments were counterbalanced across rats using a Latin-Square design. Testing was generally performed on Tuesdays and Fridays, and three-component training sessions were conducted on other weekdays. Data from training sessions were monitored to ensure a return to ICSS rates consistent with pre-drug levels to confirm drug wash-out. All testing was performed during the light phase of the light/dark cycle.

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