D54/C cells (1×104/well) was seeded in clear-bottomed 96-well black plates. After 24 hours, different concentrations of ELP-KLAK and AP1-ELP-KLAK were treated and were switch to CO2-independent culture medium supplemented with 1% Glosensor and 10% FBS. The caspase-3 sensor BLI activity was measured at different time interval using IVIS Lumina III. In order to inhibit caspase activation, the ELP-KLAK and AP1-ELP-KLAK treated cells were then treated with 20 mM Z-VAD (pan-caspase inhibitor), for an additional 1 hour and the BLI activity was obtained accordingly.

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