An RNA sequencing library was generated using the TruSeq RNA sample preparation kit (Illumina, San Diego, CA, USA). Briefly, mRNA was separated from total RNA using Oligo (dT) beads and chemically fragmented. After double-strand cDNA synthesis of fragmented mRNA, end-repair, adenylation of 3’-ends, and sequencing adapter ligation, DNA purification with magnetic beads and PCR amplification were carried out. Finally, amplified libraries were purified, quantified, and subjected to template preparation. The HiSeq2500 platform was used to generate 101-bp paired-end sequencing reads (Illumina).

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