During the years 2012–2019, a comprehensive survey of wild grapevines was conducted (Drori, Levy, et al., 2017). A total of 129 V. vsylvestris accessions were sampled in the Upper Galilee and around the Sea of Galilee in the northern part of Israel (Figure 1). Verification of the sylvestris identity of accessions and determination of their sex were performed based on their flower's structure: The male flower contains only stamens, and the female flower contains a full carpel and deteriorated stamens (Figure S1). For each plant, we sampled shoot tips or young leaves for DNA extraction. Sampling was conducted during the spring of each year, and samples were stored at −80°C until processing.

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