Fix the cells planted on the six-well plates or the frozen tissue slices in advance using the fixative solution for 10 minutes according to the manufacturer’s instructions. After being washed with PBS, cells or tissue slices were stained with SA-β-Gal kit (Abcam, Cambridge, UK) at 37°C. Microscopic observation of cells stained blue, the percentage of which reflects the number of senescence-associated cells. For frozen tissue sections, the nucleus was stained with nuclear fast red.

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