Total soluble protein content in fresh leaf materials (0.1 g) of wheat was estimated by using the 2 mL of phosphate buffer saline (pH 7.2) as described by Bradford (1976) 58 and then homogenized material was centrifuged for 10 minutes at 12000 g. The sample was incubated at room temperature for 30 minutes and measured the absorbance at 595 nm using UV-VIS spectrophotometer (Hitachi U-2910, Tokyo, Japan) and bovine serum albumin (BSA) was used as standard.

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