Total soluble protein content in fresh leaf materials (0.1 g) of wheat was estimated by using the 2 mL of phosphate buffer saline (pH 7.2) as described by Bradford (1976) 58 and then homogenized material was centrifuged for 10 minutes at 12000 g. The sample was incubated at room temperature for 30 minutes and measured the absorbance at 595 nm using UV-VIS spectrophotometer (Hitachi U-2910, Tokyo, Japan) and bovine serum albumin (BSA) was used as standard.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.