Uppermost leaves that are fully expanded were harvested at 9 to 10 am and used for the estimation of chlorophyll (a, b), total chlorophyll and carotenoid contents using the method described by Lichtenthaler (1987). 53 The supernatant was used to find out the absorbance at 645, 663, and 480 nm by using UV-VIS (Hitachi U–2910) spectrophotometer. Carotenoids contents were measured by the method of Davies (1976). 54 The chlorophyll contents and total carotenoid contents were calculated by using the following formulas.

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