Screen data were analyzed using SSMDs of percent of control, Z-score, and log(Nwell/Nplate.avg), where N represents firefly luciferase reporter activity divided by renilla luciferase activity in the corresponding well and Nplate.avg represents the average firefly reporter activity normalized to renilla luciferase control for the whole plate. The SSMD values were averaged and ranked across the entire screen. Validation data are presented as mean ± standard error of the mean (s.e.m) (n is noted in the figure legends). Statistical significance of differences was evaluated with an unpaired two-sided Student's t-test or one-way ANOVA (as indicated in the figure legends). Significance was accepted at the level of p < 0.05. Data analysis was performed using GraphPad Prism Software (GraphPad, La Jolla, CA, USA).

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