Huh-7 cells were digested with 0.25% trypsin (1 mL) for 3 min, inoculated into a 96-well plate with density of 1 × 104 cells/well, and cultured at 37 °C for 24 h. Then, Huh-7 cells were transfected and, respectively, cultured for 24 h, 48 h and 72 h. MTT solution (5 mg/mL) (Beyotime) was added to the plate with 0.02 mL per well. After further culture for 4 h under the original culture conditions, the supernatant in the wells was poured out, and 0.15 mL DMSO was added to each well, which was placed on the oscillator for 10 min to completely dissolve the crystals. The absorbance value of each well with the wavelength of 490 nm was determined by a Multiskan™ Go microplate spectrophotometer (Thermo Fisher Scientifc, Inc.).

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