Normal hepatocyte (THLE-3) and HCC cells (SK-Hep-1, Huh-7, SNU-449, HCCLM3 and MHCC97H) were obtained from American Type Culture Collection (ATCC). The whole cells were placed in DMEM medium (Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.) for routine culture and passage in a biochemical incubator at 37 °C and 5% CO2.

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