The prepared paraffin sections were stained with a TRAP staining solution (Cosmo Bio Co., Ltd., Tokyo, Japan) according to the conventional method, and then encapsulated with a cover glass and Mount-Quick Aqueous medium. Histological images were then observed under an optical microscope. The number of TRAP-positive cells that existed within 3 mm just below the growth plate and that had at least three nuclei were counted as osteoclasts. The number of osteoclasts was measured in nine sections from three mice in each group, and three sections from each mouse were selected at random.

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