To observe the femur bone resorption, 4-μm-thick slices from the mice from each group were photographed using an animal micro-CT imaging device (Skyscan: Bruker, Kontich, Belgium), and these images were reconstructed using the accompanying software (Reconstruction software, NRecon: Bruker) and analyzed using the image processing software (Analyze software, CT-Analyzer: Bruker). We measured the indicators of bone mineral density (BMD, mg/cm3), bone volume/trabecular volume (BV/TV, %), trabecular number (Tb.N, 1/μm), and trabecular separation (Tb.Sp, μm).

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