Mice were anesthetized with Ketamine and Xylazine. The spinal column was rapidly removed, and the lumbar spinal cord was retrieved. Lumbar spinal cords were flash frozen and stored at − 80 °C until processed. Frozen tissue was kept on dry ice until homogenized in Qiazol reagent (Qiagen Inc, Cat: 79306). Crude homogenate was mixed with chloroform, at a ratio of 70 μL chloroform per 350 μL homogenate, to facilitate removal of lipids, and allowed to equilibrate at room temperature for 2 min. Samples were centrifuged at 13,000×g for 15 min at 4 °C. The aqueous phase containing the RNA was transferred to a new tube. Then the following steps were completed as detailed by the manufacturer’s instructions using an RNeasy Mini Kit (Qiagen, Cat: 74104) with on-column DNAse digestion (Qiagen, Cat:79254). RNA concentration was determined via a NanoDrop spectrophotometer (ThermoFisher, Model: ND-1000). RNA integrity (RIN) for RNA-sequencing samples was measured using RNA 6000 Pico Kit in a 2100 Bioanalyzer (Agilent).

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