This protocol is extracted from research article:
16S rDNA analysis of the intestinal microbes in osteoporotic rats
Biosci Microbiota Food Health, Feb 23, 2021; DOI: 10.12938/bmfh.2020-065

The control group was comprised of sham rats, and the OP group was comprised of OVX rats, with six rats in each group. Each rat was marked with a digital ear tag.

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