BpeAQP genes were searched against the sequenced birch genome available on NCBI (https://blast.ncbi.nlm.nih.gov/Blast.cgi; accessed on 2 July 2021) and the CoGe comparative genomics platform (https://genomevolution.org/coge/). The investigations were conducted using keyword queries (“Major Intrinsic Protein” and “Aquaporin”), complemented by tBLASTn searches with conservative criteria requiring a cut-off of E-value of 1.0−5 by using the amino acid sequences of aquaporin sequences from A. thaliana (AtAQP) [2] and Olea europaea L. (OleaAQP) [5] as queries. The B. pendula AQP sequences (BpeAQP) were named according to the standardized MIP nomenclature, and each name (i.e., PIP, TIP, NIP, SIP, and XIP) was guided by sequence similarity and phylogenetic analysis with orthologs from A. thaliana, O. europaea, Lycopersicon esculentum (SlAQP; [144]) and Populus trichocarpa (PoptrAQP, [14]). Each BpeAQP gene accession is derived from the identifier of the GenBank GSS contig on which it is located. When necessary, it is subscripted with an additional lowercase letter to indicate that BpeAQP genes accessions differing only by this letter were originally assembled in the same GSS contig. The Betula AQP protein names, accession numbers, and sequences (genomic, CDS, and deduced proteins) used in this work are listed in Figure 1, Figure S1 and Table S1, respectively.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.