Human serum (H4522), 10 × phosphate-buffered saline (PBS), D-biotin (B4639), and Microspin G-25 columns (GE27-5325-01) were purchased from Millipore Sigma (St. Louis, MO). Streptavidin horseradish peroxidase conjugate (SA-HRP) (N100), TMB substrate solution (34028), 10% BSA in 1 × PBS (37525), Pierce BCA Protein Assay Kit (23225), Pierce Monomeric Avidin Agarose Kit (432040), EZ-Link Sulfo-NHS-LC-Biotin (A39257), and Halt protease inhibitor cocktail (100×) (87786) were purchased from Thermo Fisher Scientific (Waltham, MA). SARS-CoV-2 NP antibody (40143-R019) was purchased from Sino Biological (Wayne, PA). Whatman Grade 1 Chr Cellulose Chromatography paper (21427-003), glycerol (97062-452), Kanamycin sulfate (97061-600), Terrific Broth Powder (97063-418), and isopropyl-β-d-thiogalactoside (IPTG) 99% dioxane-free (AAB21149-06) were obtained from VWR (Radnor, PA). Blot paper (1703965), 4–15% Mini-PROTEAN TGX Stain-Free Precast Gels (4568083), Precision Plus Protein All Blue Prestained Protein Standards (1610373), Precision Plus Protein Unstained Standards (1610363), and Nuvia IMAC Resin (7800800) were purchased from Bio-Rad Laboratories, Inc. (Hercules, CA). Restriction enzyme NdeI (R0111S), XhoI (R0146S), BamHI (R3136S), and EcoRI (R3101S) were purchased from New England BioLabs (Ipswich, MA). Human serum samples collected from PCR-confirmed SARS-CoV-2-positive patients were purchased from Brio Systems and Curebase. These biobanked samples were collected with informed consent and IRB approval (IntegReview, CURE19-001) and provided deidentified.

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