Pearson χ2 or 2-sided Fisher exact test was used for comparison of categorical variables, and analysis of variance and Kruskal–Wallis test were used for comparison of continuous variables after testing for normality by Shapiro–Wilk test. Survivals were estimated using univariable and multivariable Cox regression analyses and the Kaplan–Meier method. Differences between groups were compared using the log rank test. Statistical significance was set at p < 0.05. Data were analyzed using the Statistical Package for the Social Sciences v25 software (IBM Corp., Armonk, NY, USA).

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