Cecal contents obtained from healthy, adult, commercial broiler chickens at slaughterhouses, and rumen samples from three fistulated bulls (body weight: 210 kg) were used as sources for isolation of LAB. One gram of each sample was dissolved in 9 mL of peptone water (0.01%) and shaken at 200 rpm for 10 min. Several dilutions from each sample (10−3 to 10−7) were prepared in a dilution tube containing peptone water (0.01%). Then, 100 µL of each diluted sample were transferred onto a plate containing MRS Rogosa agar (Oxoid CM 627, Hampshire, UK) as a selective medium for LAB [14]. Plates were anaerobically incubated at 37 °C for 48 h. Several bacterial colonies were selected from each plate and subcultured three times. A total of 80 isolates were selected and tested for Gram stain, hydrogen peroxidase and lactic acid production. The LAB strains that actively produced lactic acid were chosen for the molecular identification.

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