Total RNA was extracted from the jejunum and ileum samples using Direct-Zol™ RNA MiniPrep (Zymo Research, Irvine, USA) with TRI Reagent® (Zymo Research, Irvine, USA) according to the protocol of the manufacturer. The RNA was quantified and qualified by Qubit 4 Fluorometer (Thermo Fisher Scientific, MA, USA), and Biotek PowerWave XS2 Spectrophotometer (BioTek Instruments, Inc., USA) and 1% agarose gel. 500 ng of total RNA from each sample was used for cDNA synthesis using Prime Script RT Master Mix kit (Takara Bio Inc., Shiga, Japan).

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