On post-hatch day 21, 120 chicks from each incubation group (control group, TML group, and TMH group) were randomly selected and further subdivided into three subgroups (thermal neutral [TN], cold stress [CS], and HS) to be used in the thermal stress experiment (for a duration of 7 days). For 7 days, TN subgroups were kept at 21°C, CS subgroups were exposed to 16°C, and HS subgroups were exposed to 35°C. On days 1, 4, and 7 of the thermal stress experiments (post-hatch days 22, 25, and 28), nine chicks were randomly selected from each subgroup and their Tb and BW were recorded. After 1 day of thermal stress, ileum samples were collected from each subgroup and snap-frozen for subsequent total RNA extraction. Then, the chicks exposed to 4 and 7 days of thermal stress were humanely euthanized to collect jejunum and ileum samples (about 1 cm), which were maintained in a 10% neutral buffered formalin solution for histological morphometric evaluation.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.