Plotting was performed in figures 1, ,2,2, ,3,3, extended data figures 1, ,2,2, and and33 using ggplot2 version 3.3.1 unless otherwise noted. Comparisons for these figures were added using ggsignif version 0.6.0. Heatmaps on figures 2 and and33 were generated using pheatmap version 1.0.12. Sankey/Alluvial plots on Extended Data Fig. 1 were generated using ggalluvial version 0.12.072. Figure layouts for Figures 1, ,2,2, ,3,3, ,4,4, and Extended Data Figures 1, and and33 were generated using patchwork version 1.01 and edited in Adobe Illustrator 2021. Figure 4 and extended data figures 4 and and55 were generated with matplotlib v3.2.173. For all boxplots shown, box limitsq represent the interquartile range (IQR) with a centerline at the median. Whiskers represent the largest point ≤ 1.5 * IQR. All points are overlaid.

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