Blood samples were centrifuged at 3500 tours/min. Buffy coats were collected, aliquoted and stored at − 80 °C. DNA was extracted from buffy coat samples using the QIAamp® DNA Mini kit according to the manufacturer’s instructions. The DNA eluate was stored at − 80 °C and sent to Laboratoire d’Histocompatibilité des Cliniques Universitaire Saint Luc in Belgium, where HLA-B*57:01 typing was performed.

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