K562 (Chronic myeloid leukemia, Prof. Ufuk Gündüz Laboratory, Turkey) and CCRF-CEM (Acute lymphoid leukemia, DSMZ, Germany) cells were cultivated in 1X RPMI1640 medium (Medsantek/dist. Gibco, TR) enriched with 10% fetal bovine serum (Interlab/dist. BI, TR), 1% nonessential aminoacids (Interlab/dist. Sigma Aldrich, TR), and 1% penicillin/streptomycin (Interlab/dist. Sigma Aldrich, TR) in T25 cell culture flasks. Subculturing and drug resistance development procedures were presented in our previous work25. The same cells were used in this study after 4 passages. Based on IC50 analyses, K562 cells were classified as high-level laboratory drug resistant models with approximately 60-fold resistance to imatinib as compared to wild type K562 cells, while CCRF-CEM cells were categorized as a clinically relevant model with approximately fourfold resistance to doxorubicin as compared to their wild type progenies25,26.

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