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We used an RBD total antibody (Ig M and G) chemiluminescence assay (Siemens Healthineers), which has 100% sensitivity (≥14 days after positive polymerase chain reaction test results) and 99.8% specificity.10 An independent analysis from Public Health England confirmed the sensitivity and specificity rates.11 Remainder plasma was collected using lithium heparin gel plasma tubes and centrifuged within 2 hours at 3000 to 3400 rpm for 15 to 20 minutes. Following routine procedure, these specimens were stored and transported at 2 to 8 °C until analysis. If specimens were not tested within 2 days, then they were frozen at −20 °C until analysis could be performed.

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