In total, 5 × 104 Caco-2 cells/well were seeded in black 96-well plates with clear bottom. The second day after seeding, the spent medium was discarded and cells were treated with 100 μL/well of BUO and OMN extracts at concentrations of 10, 100, 500, or 1000 μg/mL or vehicle (C) in growth medium for 24 h at 37 °C. Experiments were carried out following previously optimized conditions [26]. More details are available in the Supplementary Material.

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