Expression of Collagens I and III, Vimentin, and α-SMA in Lung Tissues

Paraffin sections were sectioned at a thickness of approximately 4 µm. After dewaxing and hydration, 3% hydrogen peroxide was added to the sections at room temperature for 15 min for blocking the activity of endogenous peroxidases. Goat serum was added in a dropwise manner for blocking non-specific binding, and the sections were incubated at room temperature for 30 min. The serum was then removed, and the sections were incubated overnight at 4°C with the primary antibodies. On the next day, the slides were washed, treated with the secondary antibodies and the color development solution (separately), and then re-stained with hematoxylin; after dehydration and blocking, the slides were observed under a microscope. The appearance of brownish yellow areas under light microscopy (×200 magnification) was considered a positive result. The integrated optical density (IOD)/area was measured using Image-Pro Plus 7.0 software. The optical density was first corrected, and then, the IOD and area values were measured separately.

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