Phosphate buffered serology saline (PBSS) pH 7.0 [Biosciences] was used for dilution to improve reaction patterns. 22 RBC typing and antibody screening was performed by an enzyme technique 22 using Bromelain [Sigma‐Aldrich], a proteolytic enzyme used daily at a 0.1% working solution.

The five reagent RBCs (rRBCs) required were prepared from RBC packs with known phenotypes on a daily basis. RBCs were washed and prepared in saline suspension at concentrations of 1.25%‐1.45%, depending on the validated RBC concentration required for the relevant profile on the PK7300's.

An O R1R1 K+/K‐ and an O R2R2 K+/K‐ rRBC were used for antibody screening and an A1B rRBC was used to detect anti A,B high titer positive donors on the ABOa profile.

The testing of the donor plasma for its hypothetical ABO antibody/ ies (reverse group) was performed using A1 RhD‐ and B RhD‐ rRBCs on the ABOb profile.

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