The antisera for the PK7300 microplate technique had been validated in ADG to ensure potency and specificity without a compromise in sensitivity. 21

The first ABO profile (ABOa) used the following clones; anti‐ A [Bioscot‐millipore; Birma‐1], anti‐ B [Bioscot‐millipore; LB‐2], anti‐ A,B [Bioscot‐millipore; ES‐15/ES‐4], anti‐ D 1 [Diagast ‐Totem; p3x61 + p3x21223B10 + p3x290 + p3x35], and anti‐ D 2 [Immucor ‐Novaclone; D415/D175].

The second ABO profile (ABOb) used anti ‐A [Immucor‐ Novaclone; A98], anti‐ B [Immucor‐ Novaclone; B84 + B97], anti ‐A,B [Immucor‐ Novaclone; A98 + B84 + B97 + AB125], anti‐ D 1 [Bioscot‐millipore; Rum‐1], and anti ‐D 2 [Bioscot‐millipore; MS‐201].

The two anti‐ D antisera on ABOa detected DVI positive RBCs; whereas the two anti‐ D antisera on ABOb did not detect DVI positive RBCs.

The Rh phenotype profile used the following antisera clones; anti‐ C 1 [Bioscot‐millipore; MS 24] & anti ‐C 2 [Imumed‐ antitoxin; MS273], anti‐ c 1 [Bioscot‐millipore; MS33] & anti‐ c 2 [Imumed‐ antitoxin; MS35], anti‐ E 1 [Bioscot‐millipore; MS80/MS258] & anti ‐E 2 [Imumed antitoxin;MS258/906], anti‐ e 1 [Bioscot‐millipore; MS16/21/63] & anti ‐e 2 [Imumed‐ antitoxin; MS16/21/63]. A further anti ‐D antisera [Imumed‐ antitoxin; MS26/TH28] was necessary for the interpretation of the Rh phenotype (most probable Rh genotype).

The JK phenotype profile used anti ‐Jka 1 [Bioscot‐millipore; MS15] & anti‐ Jka 2 [Imumed‐antitoxin; MS15], anti ‐Jkb 1 [Bioscot‐millipore; MS 8] & anti ‐Jkb 2 [Imumed‐ antitoxin; MS 8].

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