2.1. Subjects studied
This protocol is extracted from research article:
A snapshot of ABO, RH, and JK blood group systems in modern Ireland
Health Sci Rep, Jun 12, 2021; DOI: 10.1002/hsr2.292

Donors donating for the first time to the IBTS were included in this study as this avoided any bias that would occur if using known donors from clinic call‐ups. Donors from all 26 counties were represented. Clinic locations were dependent on IBTS clinic rosters. Testing was performed over a 14 week period from August to December 2015. Venous blood was collected in Vacuette K3 EDTA anticoagulant, refrigerated overnight and on arrival to the laboratory centrifuged prior to testing at 3500 rpm (2739 g) for 5 minutes. All procedures were undertaken according to ADG standard operating procedures (SOP's). The final sample size was 3427 with 2939 donors BiI and 488 donors BoI. The Kidd profile had 3423 donors, (2935 BiI/488 BoI) with a loss of four donors (BiI) due to positive infectious disease markers. Both ABO profiles and Rh phenotype testing had been completed prior to this notification.

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