cDNA was prepared using RT2 First Strand Kit (Qiagen, Hilden, Germany) according to manufacturers’ protocol using 350ng-1000ng of total RNA as starting material. Metabolic enzymes mRNA expression levels were performed using Human Drug Metabolism: Phase I Enzymes arrays (Qiagen, Hilden, Germany), RT2 SYBR® Green qPCR Mastermix (Qiagen, Hilden, Germany) and results were acquired by Quantstudio 12k flex (Thermo Fisher Scientific, Waltham, USA).

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