We detected the expression of annexin A1, S100A9, uromodulin, and osteopontin localization in polyp samples using semiquantitative IHC analysis. The 5-μm-thick serial sections of ureteral polyp tissues were incubated with appropriate primary antibodies overnight at 4 °C before being incubated with appropriate secondary antibodies for 25 minutes at room temperature. Next, the sections were treated with peroxidase-marked streptavidin/peroxidase before being examined under an Olympus BX-51 microscope (Olympus, Japan). A semiquantitative scoring system was used to grade the immunoreaction intensity.[5] The cases were identified pathologically by a senior uropathologist blinded to the clinical outcome using the semi-quantitative H-score (0–300), including the intensity score (0 for negative, 1+ for weak, 2+ for moderate, and 3+ for strong). The intensity score was classified into 2 categories (negative or positive) to examine the concordance rate of the expressions of markers between different sites of the same organ.

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