2.5.5. Studying the ROS Scavenging Potential of the Isolated Metabolites and the Chloroform Fraction

ROS scavenging potential was assessed, as formerly stated in previous work from our laboratories [20]. In a 96-well clear plate, the pure metabolites (1–10 µM) or Fraction I (1–10 µg/mL) in MeOH was added to a DPPH (240 µM) solution in MeOH/tris (1:1 v/v). For the control (C), MeOH was utilized instead of the fraction or metabolites. DPPH was directly prepared before adding to the plate. The absorbance was estimated every minute at 520 nm for 10 min using a SpectraMax® M3-Monochromator plate reader.

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