Petals of the two cultivars were collected at the same time when flowers began to bloom. Three frozen petals from three different plants (numbered P1 to P3/W1 to w3) were randomly selected for RNA extraction in each replicate. Total RNA was extracted with the EasyPure® Plant RNA Kit (TransGen Biotech Co., Ltd.) following the manufacturer’s instruction and DNA was removed with RNase-free DNase. After concentration and quality of RNA were detected, mRNA was fragmented into small pieces, and first-strand cDNA was synthesized with a random hexamer primer and M-MuLV Reverse Transcriptase. Then the second-strand cDNA was synthesized with DNA Polymerase I and RNase H, and the remaining overhangs were then converted into blunt ends. Finally, PCR was performed with Phusion High-Fidelity DNA polymerase and universal PCR primers, Index (X) Primer, and PCR products were purified by the fdAMPure XP system.

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