Flow cytometry analysis of apoptosis
This protocol is extracted from research article:
RBM5-AS1 promotes radioresistance in medulloblastoma through stabilization of SIRT6 protein
Acta Neuropathol Commun, Jul 5, 2021; DOI: 10.1186/s40478-021-01218-2

Apoptotic response was evaluated 48 h after exposure to 8 Gy of X-rays. In brief, cells were washed and resuspended in the Annexin Binding Buffer. The cell suspension was added with Annexin V conjugated with fluorescein isothiocyanate (FITC; Sigma-Aldrich) and incubated for 15 min at 4 °C. Afterwards, the cells were stained with propidium iodide (PI; Sigma-Aldrich). Apoptotic cells were immediately analyzed using the FACScantoII cytometer with the FlowJo 10.2 software (BD Bioscience, San Jose, CA, USA).

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