Establishment of radioresistant cell lines
This protocol is extracted from research article:
RBM5-AS1 promotes radioresistance in medulloblastoma through stabilization of SIRT6 protein
Acta Neuropathol Commun, Jul 5, 2021; DOI: 10.1186/s40478-021-01218-2

Radioresistant DAOY-IR cells were developed as described previously [43]. Briefly, DAOY cells were repeatedly exposed to 4 Gy of X-rays. After treatment with a cumulative dose of 80 Gy, the surviving cell clones, namely DAOY-IR, were recovered and expanded.

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